Journal article
Development of RFLP markers for barley
Publication Details
Authors: | Jahoor, A.; Backes, G.; Graner, A.; Herrmann, R.; Fischbeck, G. |
Publication year: | 1991 |
Journal: | Plant Breeding |
Pages range : | 73-76 |
Volume number: | 107 |
ISSN: | 0179-9541 |
DOI-Link der Erstveröffentlichung: |
Abstract
A PstI-based genomic library from barley DNA was screened for RFLPs in the three relatively-distant cultivars 'Alexis' (2-row spring type), 'Igri' (2-row winter type) and 'Mammut' (6-row winter type), digested with BamHI, EcoRI and HindIII. 50 {\%} of the 108 DNA fragments studied represented single-copy sequences, 29 {\%} low-copy and 21 {\%} repetitive sequences. The DNA probes were assigned to discrete barley chromosomes with the aid of wheat/barley addition lines. 80 {\%} of the single- and low-copy sequences hybridized with both barley and wheat DNA, whereas most repetitive sequences gave signals only with barley DNA.
A PstI-based genomic library from barley DNA was screened for RFLPs in the three relatively-distant cultivars 'Alexis' (2-row spring type), 'Igri' (2-row winter type) and 'Mammut' (6-row winter type), digested with BamHI, EcoRI and HindIII. 50 {\%} of the 108 DNA fragments studied represented single-copy sequences, 29 {\%} low-copy and 21 {\%} repetitive sequences. The DNA probes were assigned to discrete barley chromosomes with the aid of wheat/barley addition lines. 80 {\%} of the single- and low-copy sequences hybridized with both barley and wheat DNA, whereas most repetitive sequences gave signals only with barley DNA.
Keywords
Addition lines, Additions-Linien, Barley, Development, Gerste, Hordeum vulgare, Igri, Marker-Entwicklung, RFLP marker, RFLP-Marker
